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Literature summary extracted from

  • Muesing, R.A.; Porter, J.W.
    Fatty acid synthase from pigeon liver (1975), Methods Enzymol., 35, 45-49.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.85 dithiothreitol stabilizes Columba sp.
2.3.1.85 foaming leads to surface inactivation Columba sp.
2.3.1.85 low ionic strength leads to dissociation of native enzyme and inactivation Columba sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.85 additional information
-
 additional information Km-values for various partial activities of fatty acid synthetase Columba sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.85 soluble
-
 Gallus gallus
-
-
2.3.1.85 soluble
-
 Rattus norvegicus
-
-
2.3.1.85 soluble
-
 Columba sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.85 450000
-
-
 Columba sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ Gallus gallus multifunctional enzyme, involved in animal fat synthesis palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ Rattus norvegicus multifunctional enzyme, involved in animal fat synthesis palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ Columba sp. multifunctional enzyme, involved in animal fat synthesis palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.85 Columba sp.
-
 pigeon
-
2.3.1.85 Gallus gallus
-
-
-
2.3.1.85 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.85 homogeneity Gallus gallus
2.3.1.85 homogeneity Rattus norvegicus
2.3.1.85 homogeneity Columba sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O reaction mechanism Gallus gallus
a
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O reaction mechanism Rattus norvegicus
a
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O reaction mechanism Columba sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.85 liver
-
 Gallus gallus
-
2.3.1.85 liver
-
 Rattus norvegicus
-
2.3.1.85 liver
-
 Columba sp.
-
2.3.1.85 mammary gland
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.85 0.078 0.1 palmitate Columba sp.
2.3.1.85 1.12 1.4 NADPH, 30°C Columba sp.

Storage Stability

EC Number Storage Stability Organism
2.3.1.85 -20°C, at least a month in the presence of DTT Columba sp.
2.3.1.85 -20°C, under nitrogen at least 1-2 months Columba sp.
2.3.1.85 4°C, 90% loss of activity in 8 days with 40% dissociation of native enzyme, incubation at 38°C with 10 mM DTT restores activity Columba sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
-
 Gallus gallus palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O in the absence of NADPH the product is triacetic acid lactone ?
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
-
 Rattus norvegicus palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O in the absence of NADPH the product is triacetic acid lactone ?
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ specific for malonyl-CoA, acetyl-CoA can be replaced by propionyl-CoA or butyryl-CoA Columba sp. palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O in the absence of NADPH the product is triacetic acid lactone ?
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ multifunctional enzyme, involved in animal fat synthesis Gallus gallus palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ multifunctional enzyme, involved in animal fat synthesis Rattus norvegicus palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ multifunctional enzyme, involved in animal fat synthesis Columba sp. palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.85 25
-
 assay at Gallus gallus
2.3.1.85 25
-
 assay at Rattus norvegicus
2.3.1.85 25
-
 assay at Columba sp.
2.3.1.85 30
-
 assay at Gallus gallus
2.3.1.85 30
-
 assay at Rattus norvegicus
2.3.1.85 30
-
 assay at Columba sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.85 25
-
 50% loss of activity in dilute, mildly alkaline solution within 3.5 h, high ionic strength together with DTT reactivates Columba sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.85 6.8
-
 assay at Gallus gallus
2.3.1.85 6.8
-
 assay at Rattus norvegicus
2.3.1.85 6.8
-
 assay at Columba sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.85 8.4
-
 inactivation, decreased by 0.2 M KCl or 0.02 mM NADP(H) Columba sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.85 4'-phosphopantetheine
-
 Gallus gallus
2.3.1.85 4'-phosphopantetheine
-
 Rattus norvegicus
2.3.1.85 4'-phosphopantetheine
-
 Columba sp.
2.3.1.85 additional information no requirement for FMN Gallus gallus
2.3.1.85 additional information no requirement for FMN Rattus norvegicus
2.3.1.85 additional information no requirement for FMN Columba sp.
2.3.1.85 NADH requirement, 10% as effective as NADPH Columba sp.
2.3.1.85 NADPH
-
 Gallus gallus
2.3.1.85 NADPH
-
 Rattus norvegicus
2.3.1.85 NADPH
-
 Columba sp.